Innovative Genomics & Bioinformatics Core

Innovative Genomics & Bioinformatics Core (IGBC)

IGBC Mission

Provide a total solution of genomics research from consultancy to experiments to data analysis. Our goal is to get a project from “sample to figure.”

Equipment

Illumina NextSeq 2000

Illumina NextSeq 2000

P1

P2

P3

Read Legnth

100bp

100bp

100bp/200bp

Read Number

100 million

500 million

1.2 billion

Sequencing Cost

$920

$1,475

$3,151/$4,633


  • Bulk RNA-seq
  • Single-cell RNA-seq
  • Whole genome sequencing
  • P1, P2, & P3 reagents available
  • Cost examples using P3 reagents:
    • $3151 per run (100bp)
      • 5 10x Genomics scRNA-seq samples
        • $630 per sample
      • 60 bulk RNA-seq samples
        • $52 per sample
  • Turnaround time:
    • 1 day

10x Genomics Chromium iX Controller


  • Library prep for:
    • Single-cell RNA-seq
    • Single-cell ATAC-seq
  • Lead time: 2 weeks
  • Minimal requirement:
    • Cell/nuclei suspensions 1,000 cells/μL >20μL
  • • 10,000 target cells per sample
  • Cost:
    • $1,878 scRNA-seq

Aligent TapeStation 4200


  • • RNA quantification, assessment of RNA integrity, DNA quantification, Fragment size analysis
  • Cost:
    • HS 1000 & HS 5000:
      • $5 per lane, $6.50 per ladder
    • HS RNA:
      • $4 per lane, $6.50 per ladder
    • $1 per lane if using non-IGC reagents

Qubit Flex Fluorometer


  • Quickly quantifies dsDNA, ssDNA, RNA, or protein
  • Quantify up to 8 samples at a time
  • Cost:
    • $1 per sample

Data Analysis

Sequencing Data Analysis

Sequencing Data Analysis

  • Cost:
    • $20 per hour
      • Special price for sequencer users
    • Turnaround time:
      • 2 days for standard NGS analysis
      • For customized analyses, time varies
    • Examples:
      • Differential gene expression
      • Copy number alterations
      • Functional annotation of genes
      • Annotation of SNVs & indels
      • Splicing event detection
      • Etc.

Training & Consultations

  • Cost:
    • Training for standard NGS analysis: $50 an hour
    • Consultations: $20 an hour
    • Special discounts for sequencer users

Contact, Location & Reservation Information

Contacts

Co-Director:
Jun Xia, PhD
junxia@creighton.edu

Co-Director:
Yusi Fu, PhD
yusifu@creighton.edu

Core Manager:
Aaron Mohammed, MS
aaronmohammed@creighton.edu

 

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Location

Criss III, Second Floor, Room 259

Reservations

Sequencer Reservations
Aaron Mohammed, MS
aaronmohammed@creighton.edu

TapeStation Reservations
Please use the Reservation System to schedule a reservation.

Training/Consultation Reservations
Aaron Mohammed, MS
aaronmohammed@creighton.edu