What is Confocal Microscopy?
Visit Zeiss or Olympus for great confocal tutorials.
Projected and Stereoscopic Images
How to make pictures and movies with depth!
Large Area Imaging
How to use the automated stage to make tiled images.
Protein 'Co-Localization' (within 200nm)
How to show two fluorescently labeled proteins are 'co-localized.'
Protein Co-Localization (within 5 to 10 nm)
Förster resonance energy transfer (FRET) techniques yield information about functional distances between two proteins.
Fluorescence Spectra Generation
Generate emission spectra for individual fluorophores which can be used for separation of overlapping.
Generate emission spectra for individual fluorophores which can be used for separation of overlapping.
Quantitative Fluorescence
Quantification of observed differences in fluorescence intensity.
Physiology Experiments
Redox imaging of living cells.
Magnesium Green measurement of changes in ATP concentration in living cells.
Multiple probes can measure the production of reactive oxygen species (ROS) in living cells. These probes are fluorescent only when oxidized and are sensitive to a wide variety of ROS. LeAnn Tiede, Creighton University Dept. of Biomedical Sciences.
Multicolor Imaging (Labeling of Cochlear Neurons Using 6 Different PTIR® Dyes)
Dr. Bernd Fritzsch, University of Iowa, Department of Biology, Dr. Michael Nichols, Creighton University Department of Biomedical Sciences and Physics.
3D Reconstruction of a Staphylococcus Aureus Biofilm
Ethan Mann, University of Nebraska Medical Center, Department of Microbiology and Immunology.
Alpha-actinin Labeling in Smooth Muscle Cells
Dr. Yan Xie, Creighton University Pharmacology
Nile Red Stained Snake Skin
Laxmi Fogueri and Dr. Somnath Singh, Creighton University Pharmacy
Convallaria Autofluorescence
Dr. Heather Jensen-Smith, Creighton University Department of Biomedical Sciences
Afferent and Efferent Innervation of the Cochlea
Dr. Bernd Fritzsch, University of Iowa, Department of Biology
Nuclear Co-Localization of Poly A Binding Protein and hnPRC in Heat Shocked HeLa Cells
Korley Leland, Dana Salerno, Dr. Kristen Drescher, Creighton University Department of Medical Microbiology and Immunology
Streptoccus Pneumoniae Phagocytized by Alveolar Macrophages
Adam Pitz, Dr. Martha Gentry-Nielsen, Creighton University Department of Medical Microbiology and Immunology
Cerebellum Crosssection
Sarah Hake, Dr. Annemarie Shibata, Creighton University Department of Biology
