Confocal and Widefield imaging of fluorescently labeled specimens using a Nikon Ti-E inverted microscope with a Yokagawa Spinning Disc for confocal imaging and Enhanced Resolution.
Lasers
- 405 nm for Dapi, Hoechst, CFP etc.
- 488 nm for FITC, Alexa Fluor 488, GFP etc.
- 561 nm for rhodamine, Texas red, mRFP1, Alexa Fluor 546, 555, 568, 594 etc.
- 640 nm for Cy5 etc.
- 594 nm for Alexa 594
Microscope
Nikon Ti-E confocal microscope, with the following objectives
- Plan Apochromat Lambda 10x Objective Lens, N.A. 0.45, W.D. 4.0mm
- Plan Apochromat Lambda 20x Objective Lens, N.A. 0.75, W.D. 1.0mm
- Super Plan Fluor ELWD 20x Objective Lens, N.A. 0.45, W.D. 6.9-8.2mm
- Apochromat Lambda S 40x Water Immersion Objective Lens, N.A. 1.25, W.D. 0.16-0.2mm
- Super Fluor 40x Oil Immersion Objective Lens, N.A. 1.3, W.D. 0.22mm
- Super Plan Fluor ELWD 40x Objective Lens, N.A. 0.6, W.D. 2.8-3.6mm
- Plan Apochromat Lambda 60x Oil Immersion Objective Lens, N.A. 1.4, W.D. 0.13mm
- Plan Apochromat Lambda 100x Oil Immersion Objective lens, N.A. 1.49, W.D. 0.13
System Resources
- Imaging of most standard fluorophores.
- Simultaneous fluorescent and DIC imaging of most samples.
- Heated, motorized stage for live cell imaging.
