Research Services

Integrated Biomedical Imaging Facility


Michael Nichols
Phone: 402.280.2225

Ann Cavanaugh
Assistant Director
Phone: 402.280.1877

Anthony Stender

Integrated Biomedical Imaging Facility

The Integrated Biomedical Imaging Facility (IBIF) at Creighton University is a core facility focused on providing investigators with access to state-of-the art techniques in light microscopy that are critical to imaging experiments in the biomedical, chemical, and materials sciences.  Although based in Omaha, we are happy to connect with investigators outside of the Omaha area to discuss their needs and interests in terms of imaging and imaging related services.

IBIF was established in 2004 by Dr. Michael Nichols (Physics) and Dr. Richard Hallworth (Biomedical Sciences).  In our first twenty years, IBIF has been acknowledged in more than 100 peer-reviewed journal articles and 2 book chapters.  When it was founded, IBIF was the first core imaging facility in the state of Nebraska to offer multiphoton, confocal, and other advanced light imaging techniques.  As we enjoy our twentieth anniversary, we continue to build on our early history and aim to the instrumental capabilities that enable our users to succeed at the work they feel called to pursue.

Imaging Capabilities

We offer the opportunity to perform a vast array of experiments that rely on imaging samples with fluorescence and visible light microscopy techniques.  A partial listing of our capabilities is provided below:

  • Confocal Fluorescence
  • Fluorescence Lifetime Imaging (FLIM)
  • Widefield Fluorescence
  • Forster Resonant Energy Transfer (FRET)
  • Multiphoton
  • Single Molecule Detection
  • Second Harmonic Generation (SHG)
  • Super-Resolution
  • Total Internal Reflection (TIRF)
  • Near-Infrared Imaging
  • Brightfield
  • Live Cell and Small Animal Imaging
  • Differential Interference Contrast (DIC)
  • Real-Time and Extended Timelapse Imaging
  • Phase Contrast
  • Z-Stacks and Tiled-Scans

Acknowledging IBIF

Please remember to acknowledge IBIF and refer to our RRID in your publications.  We recommend that you use the following statement to acknowledge IBIF:

This research was partially conducted at the Integrated Biomedical Imaging Facility at Creighton University, Omaha, NE (RRID:SCR_023806). This facility is supported by the Creighton University School of Medicine and grants GM103427 and GM139762 from the National Institute of General Medical Science (NIGMS), a component of the National Institutes of Health (NIH). The facility was constructed with support from grants from the National Center for Research Resources (RR016469) and the NIGMS (GM103427). This investigation is solely the responsibility of the authors and does not necessarily represent the official views of NIGMS or NIH.

About IBIF

Creighton University is a member of the Great Plains IDeA-CTR, making IBIF a part of NIH’s IDeA Networks of Biomedical Research Excellence.  Other member institutions of the IDeA network are encouraged to contact us with their questions about our services and special rates for fellow IDeA members. IBIF is also a partner in the Nebraska Imaging Core Facility with the University of Nebraska Medical Center.

IBIF is supported financially by the Creighton University School of Medicine, the Nebraska INBRE program, and by user fees.   The facility is located on Creighton’s Omaha campus in the Dr. C.C. and Mabel L. Criss Health Sciences Complex II and III.

IBIF now has a Research Resource ID:  RRID:SCR_023806.  Please include this number when acknowledging work completed at IBIF.  An RRID works similar to an ORC-ID and assists us in tracking research performed at IBIF.